Virology Journal | Abstract | RNA binding properties of the US11 ...

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Abstract (provisional) Background The protein encoded by the Us11 gene of herpes simplex viruses is a dsRNA binding protein which inhibits protein kinase R activity, thereby preventing the interferon-induced shut <a href="http://www.splendidgucci.com/"><strong>handbag uk</strong></a> down of protein synthesis following viral infection. Us11 protein is not essential for infectivity in vitro and in mice in herpes simplex virus type 1 (HSV1), however this virus has a second, and apparently more important, inhibitor of PKR activity, the gamma134.5 protein. Recently sequenced simian simplexviruses <a href="http://www.splendidgucci.com/coach-handbags-c-2.html"><strong>coach handbag</strong></a> SA8, HVP2 and B virus do not have an ORF corresponding to the gamma134.5 protein, yet they have similar, or greater, infectivity as HSV1 and HSV2. Methods We have expressed the US11 proteins of the simplexviruses HSV1, HSV2, HVP2 and B virus and measured their abilities to bind dsRNA, in order to investigate possible <a href="http://www.splendidgucci.com/coach-handbags-c-2.html"><strong>coach handbags outlet</strong></a> differences that could complement the absence of the gamma134.5 protein. We employed a filter binding technique that allows binding of the Us11 protein under condition of excess dsRNA substrate and therefore a measurement of the true Kd value of Us11-dsRNA binding. Results and conclusions The results show a Kd of binding in the range of 0.89 nM to 1.82 nM, with no significant difference among <a href="http://poze.hanuancutei.com/displayimage.php?pos=-344"><strong>Finance Articles, Loans, GHD people who find | Finance Articles ...</strong></a> the four Us11 proteins. The complete article is available as a provisional PDF. The fully formatted PDF and HTML versions are in production.