P-gp, GSTπ and Topo Ⅱ in gastric carcinoma and its clinical significance
ceforadrug-resisrantformOftOpoisomeraseIlJBio, (7hem, l989: 264:164 Il ~ 2212SmYS, SubJM, AhnSH PournyCetalExpre ~ ion | 1fmdr a 1geneinhumanbreastprimaryturllorsandnletastases only, St (ricerRes7)-eal, 1993:26 (3): 267, respectively, on a slide show HID-AB-PAS staining and HP's heart Lugen Fu Chen Jianying Wang Ji climbing Keywords biopsy; staining CLC R361.2 Document code B Article ID 1001 ~ 7399 (2002) 04-0392-01 present HIDAB-PAS and Helicobacter pylori (Hp) infection has a special routine endoscopic biopsy diagnosis of contents The former can detect properties of epithelial mucus, which is easy to find HP. We try to make two slices with a special dye to obtain good results are reported as l Materials and methods 1.1 Materials from our department in May 2000 to 2001 April endoscopic biopsy specimens of 2605 cases, l [j% neutral formalin fixation, dehydration, transparent, dip wax, embedded, sliced thick 4 ~ 5m, of which 1543 cases were done with two slides HID -AB-PAS and HP special dye, 1062 cases were made on glass slides in a special dye 1.2 of the two reagents prepared HII) for the high iron diamine,
tresor paris bracelet, AB solution of Alcian blue dye for the pH2.5, PAS 0.5% dye solution and Schiff periodic acid oxidation solution, HP 2% methylene blue dye (methylene blue) 1.3 staining method will be ready to do HP and HID-ABPAS the same organization of two wax fishing on a slide, fishing is generally the first to do HID-AI% PAS central wax on the slide, and then fishing do HP's wax 2 ~ 3cm at its bottom. the first drops of high-speed rail in the first two _ = a piece of tissue amine on overnight, AB Day 2 drops of liquid, then a PAS stain, do not have to worry about HP dye will do next to the organization of pre-infected with color, such as HID-AGPAS done after staining the slides a little dry (key steps, such as slide quit, the next step will be spread to methylene blue dye has been dyed a good HID-AB-PAS organization, come to naught) and finally dropping 2% methylene blue in the first two organization about l0min, running water, dehydrated transparent Sealing 2 results with a slide made the above two kinds of special dye without disturbing each other, their color clarity, HP slice of pure blue (Figure 1), HID-AB-PAS were black, blue, red ( Figure 2), and the effect of using two glass slides to do exactly the same. Received Date: 200l a 09-26 Author: seven new Mirage Pudong People's Hospital, Department of Pathology, 201 200 Ⅸ Author: Ji heart, male, 48 years old, technician in charge . Tel: (021) 58981990-2l06'-I,, ', 3 Discussion Figure 1 Helicobacter pylori quiet f] methylene blue stain × l000 liver Pi Figure 2HII)-AB-PAS staining × 400 endoscopic biopsy specimens of two kinds of the separately in a special dye on the slide, you can save when you ask, procedures, slides, labels,
moncler inverno, etc., but the actual work, often encounter staining interference and other issues we have gone through a lot of practice, and explore the use of methylene blue HP did replace the original special stained with Giemsa dye, not only to the addition of eosin after the Society for ingredients to make more clear blue stain,
UGGs kopen online, clear, and red with the HID-ABPAS neutral mucopolysaccharide were more obvious. methylene blue is one kind of quinone imine dye with two chromophores, one for the mouth of Indian amine, and the other for the quinone-type benzene ring, so strong staining; and HID-AB-PAS staining of the high iron diamine, Alcian blue ,
moncler zürich, ~ hiff reagents were diamine salts,
moncler jassen, water-soluble copper cyanide and benzene, methane titanium sub-class. so staining is weak, a long reaction time, which can be used methylene blue because they do not cover small, fi, infected with HID-AB -PAS tissue sections:
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